《植物生理学报》 2012, 48(10): 949-957

植物丙酮酸磷酸双激酶(PPDK)研究进展

王真梅, 李海霞, 何莹, 曾汉来*

华中农业大学植物科学技术学院, 农业部长江中游作物生理生态与耕作重点实验室, 武汉430070

通信作者：曾汉来；E-mail: zenghl@mail.hzau.edu.cn；Tel: 027-8728-3465

摘　要：

丙酮酸磷酸双激酶(PPDK)是C4植物和景天科酸代谢(CAM)植物光合作用的关键酶, 催化形成固定CO2的初始分子受体磷酸烯醇式丙酮酸(PEP)。本文重点比较了植物的PPDK基因结构及分子进化关系, 综述了PPDK在C4植物和C3植物中的功能、PPDK的调控机理、PPDK在胁迫条件下的功能以及转PPDK基因等在近年来的研究进展。

关键词：丙酮酸磷酸双激酶; 活性调控; 胁迫; 基因工程

收稿：2012-05-24   修定：2012-05-24

资助：湖北省自然科学基金重点项目和中国博士后科学基金项目(20100480914)。

Advances in Plant Pyruvate, Orthophosphate Dikinase

WANG Zhen-Mei, LI Hai-Xia, HE Ying, ZENG Han-Lai*

Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, Ministry of Agriculture, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: ZENG Han-Lai; E-mail: zenghl@mail.hzau.edu.cn; Tel: 027-8728-3465

Abstract:

Pyruvate, orthophosphate dikinase (PPDK) is a cardinal enzyme of the C₄ and CAM pathway. Its role in C₄ photosynthesis is to catalyze the regeneration of the CO₂ acceptor PEP. This chapter addresses the comparisons among plant PPDK genes and the evolution and phylogenetic distribution of PPDK. The current stateof-knowledge on PPDK functions in C₄ and C₃ plants and the regulation of PPDK are described. Also reviewed are the role of PPDK in stress and transgenic plants expressing PPDK.

Key words: pyruvate, orthophosphate dikinase; activity regulation; stress; genetic engineering